Expression changes of IRS-1/2 and PI3K in GDM adipocyte cells after anthropogenic Omentin-1, Vaspin transfection
PAN Baolong
MA Runmei
Abstract：Objective To observe the expression changes of insulin receptor substrate-1/2 ( IRS-1/2) and phosphati-dy inositol 3 kinase (PI3K) in gestational diabetes mellitus (GDM) adipocyte cells after anthropogenic Omentin-1, visceral adipose tissue-derived serine protease inhibitor ( Vaspin) transfection.Methods Recoveried, extended and differentiated GDM preadipocyte cells were prepared, and then we built Omentin-1, Vaspin overexpression carrier, and transfected the extended adipose cells with different overexpression levels (1.0, 2.5 and 5.0μg).Meanwhile, no transfection group was taken as the contrast.Using Q-PCR to detect the mRNA expression of Omentin-1, Vaspin, IRS-1/2 and PI3K (P85a), using Western blotting to detect protein expression of Omentin-1, Vaspin, IRS-1/2 and PI3K ( P85a) as well as IRS-1/2 phosphorylation levels, using [3H]-2-deoxidation-D-glucose uptake assay to detect the rates of glucose uptake in different transfection groups.Results With the increasing Omentin-1 expression, the mRNA and protein expression of IRS-1 and PI3K(P85a) were increased, but those of IRS-2 had no significant changes, IRS-1 phosphorylation increased obviously, IRS-2 phosphorylation had no significant changes, and the glucose uptake rate increased significantly.With the increasing Vaspin expression, all of the mRNA, protein and phosphorylation in IRS-1/2 and PI3K(P85a) had no significant changes, so did the glucose uptake rates.Conclusion In GDM adipocyte cells after anthropogenic Omentin-1 transfection, IRS-1, PI3K( P85a) and glucose uptake rates were increased, but no changes in those of GDM adipocyte cells after anthropogenic Vaspin transfection.
Keywords：gestational diabetes mellitusOmentin-1visceral adipose tissue-derived serine protease inhibitorgene transfectioninsulin resistanceinsulin receptor substrate-1insulin receptor substrate-2phosphatidy inositol 3 kinase
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 9-12 )