Effect of 1,25(OH)2 D3 on migratory,invasive ability and expression of MMP-2,9 and 14 of human pancreatic cancer cell line PANC-1
YANG Sheng
LIU Shengcai
CHEN Wenhua
HU Tengteng
LIN Jun
Abstract：Objective To investigate the effect of 1,25-dihydroxyvitamin D3 [1,25(OH)2 D3 ]on invasive and migra-tory abilities of human pancreatic cancer cell line PANC-1 and the changes in the expression of matrix metalloproteinases (MMP)-2,9 and 14.Methods The routinely cultured human pancreatic cancer cell line PANC-1 in vitro was treated with different concentrations of 1,25(OH)2 D3 (0,25,50,75 and 100 μmol/L).After 24 hours,the abilities of migra-tion and invasion in vitro in each group were detected by Transwell migration and invasion assays.After 48 hours,the pro-tein expression of MMP-2,9 and 14 in each group was measured by Western blotting.Results After intervention for 24 h with different concentrations of 1,25(OH)2 D3 (0,25,50,75 and 100 μmol/L),every field′s invasive cells in each group were (46.20 ±0.84),(30.80 ±2.28),(18.80 ±1.64),(13.20 ±2.39)and (5.20 ±1.30)/HP;and the migratory cells in each group were (66.03 ±1.25),(55.82 ±1.65),(41.43 ±1.51),(19.86 ±1.45)and (10.23 ±1.95)/HP,respectively.The migratory and invasive cells were decreased with the increase of concentration,and the difference was statistically significant (all P <0.05).After intervention for 48 h with different concentrations of 1,25 (OH)2 D3 , MMP-2,9 and 14 protein expression showed a declining trend with the increase of concentration,and the difference was statistically significant (all P <0.05).Conclusion 1,25(OH)2 D3 can inhibit the invasion and migration of PANC-1 cells through down-regulating the expression of MMP-2,9 and 14.
Keywords：pancreatic carcinomacell invasioncell migration125-dihydroxyvitamin D3matrix metalloprotein-ase
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 17-19 )