Inhibitory effect of sodium ferlate on proliferation of human colon cancer Lovo cells in vitro and in vivo

JIN Jian; ZHAO Qing

doi:10.3969/j.issn.1002-266X.2016.34.003

Abstract：Objective To investigate in vitro and in vivo inhibitory effects of sodium ferlate on the proliferation of hu-man colon cancer Lovo cells as well as the relevant mechanism.Methods Lovo cells were divided into two groups:the observation group which was co-cultured with sodium ferlate with the final concentrations of 0.125,0.25,0.5 and 1.0 mg/mL,and the control group without any sodium ferlate.The optical density of Lovo cells was determined by MTT.The vitali-ty index was calculated.The effects of sodium ferlate on the cell cycle and apoptosis of Lovo cells were analyzed by flow cy-tometry.Fourteen nude mice bearing Lovo cells were divided into two groups:the observation group which was injected with 50 μL sodium ferlate with the concentration of 0.5 mg/mL,and the control group which was injected by the same amount of normal saline,every other day,for 7 times and the tumor volume was measured before and after medication.The expres-sion of Bcl-2 and pro-Caspase-3 in Lovo cells and tumor tissues was determined by Western blotting.Results The cell vitality indexes were respectively 81.23 ±6.58,75.69 ±6.34,62.18 ±3.29 and 58.39 ±2.69 in the observation group after 24-hour treatment of sodium ferlate with the final concentrations of 0.125,0.25,0.5 and 1.0 mg/mL.The cell pro-portion ratio of G1 phase was decreased,and was decreased in the S phase (all P <0.05),meanwhile,the cell apoptosis rate was increased (P <0.05).Compared with the control group,the tumor volume decreased in the tumor-bearing nude mice of the observation group (all P <0.05);and the expression of Bcl-2 and pro-Caspase-3 was decreased in the Lovo cells and tumor tissues of nude mice in the observation group (all P <0.05).Conclusion Sodium ferlate may inhibit the expression of Bcl-2,activate caspase-3 and promote the apoptosis,and thus inhibit the proliferation of Lovo cells.