XIAP gene inhibited by siRNA and its effect on apoptosis and cell cycle of HeLa cells

TANG Weiwei; ZHENG Hong; LI Guohui; WANG linlin

doi:10.3969/j.issn.1002-266X.2016.33.007

Abstract：Objective To explore the inhibitory effect of small interfering RNA(siRNA)silencing human X-linked inhibitor of apoptosis protein (XIAP)gene on cell cycle and apoptosis of cervical HeLa cells.Methods DNA template coding XIAP specific siRNA was designed and synthesized.The Hela cells were divided into 4 groups:the observation group (pGPU6 /GFP/Neo /XIAP-siRNA-transfected cells),blank group (not added with siRNA and transfection reagent), negative control group (NC group,transfected by disrupted siRNA)and transfection reagent control group (Mock group, added with transfection reagents and without siRNA).The XIAP-siRNA was transfected into HeLa cells.The expression level of XIAP mRNA was assayed by RT-PCR.The apoptosis rate and cell cycle distribution were analyzed by flow cytome-try.Results Significant difference was found in the expression of XIAP mRNA at 48 and 72 h after transfection between the observation group and blank group (all P <0.05),but no significant difference was found among the blank group, Mock group and NC group (all P >0.05).In the observation group,the interference rates of HeLa cells transfected with XIAP-siRNA at 48 and 72 h were 27% and 59%.The apoptotic rate of the observation group was higher than that of the blank group (all P <0.05).Significant difference was found in the apoptosis rate of the observation group between the transfection of 48 h and 72 h (P <0.05).Compared with the blank group,the cells in the G0-G1 phrase increased and then decreased in the S phrase at 48 and 72 h after transfection (all P <0.05).Condnsion Specific XIAP-siRNA can ef-fectively silence the XIAP gene of cervical cancer Hela cells,inhibit the proliferation and promote the apoptosis.