Effects of FrzA gene transduction on Wnt signaling pathway expression and apoptosis of cardiomyocytes in rats with ischemia-hypoxia in vitro
LI Huayin
TAO Jing
MA Yitong
LIU Fen
CHEN Bangdang
Abstract：Objective To investigate the effects of recombinant adeno-associated virus type 9 (rAAV9)carrying FrzA gene transduction in inhibiting Wnt signaling pathway activity and on myocardial apoptosis of cardiomyocytes in rat models with ischemia-hypoxia.Methods The myocardial cells of SD rats were isolated and cultured.A recombinant AAV9 vector carrying FrzA gene was transfected into cardiomyocytes and then a hypoxia model was created.We divided them into four groups:control group,ischemia-hypoxia group,ischemia-hypoxia +empty virus (rAAV9-CMV-GFP)group (empty virus group)and ischemia-hypoxia +rAAV9-CMV-FrzA group (FrzA group).RT-PCR was used to detect the expression of tar-get gene FrzA,and Western blotting was applied to detect the levels of Dvl-1,β-catenin and c-Myc as well as expression of apoptosis-related proteins Bcl-2 and Bax.Results On the fifth day after virus transfection,the FrzA target gene was highly expressed in cardiomyocytes (all P <0.05).The expression of Wnt signaling pathway molecules Dvl-1,β-catenin and c-Myc was significantly higher in the ischemia-hypoxia group,empty virus group and FrzA group,and the ratio of Bax/Bcl-2 was also higher than that of the control group (all P <0.05).The expression of Wnt signaling pathway molecules Dvl-1,β-catenin and c-Myc was significantly lower in FrzA group,and the ratio of Bax/Bcl-2 was also lower than that of the ische-mia-hypoxia group and empty virus group (all P <0.05).Conclusion rAAV9 carrying FrzA gene can effectively inter-vene the Wnt signaling pathway and inhibit its activity and thus reduce the apoptosis of cardiomyocytes.
Keywords：cardiomyocytesischemia-hypoxiarecombinant adeno-associated virus type 9FrzA geneWnt signa-ling pathwayapoptosis
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 8-10 )