Effects of ischemic preconditioning on plasma endothelin in rats with tourniquet-induced limb ischemia reperfusion injury
ZHANG Xiezhuo
YAN Bin
LIU Liguo
HA Silu
Yilihamu Tuoheti
Abstract：Objective To observe the changes of plasma endothelin ( ET) in rats with tourniquet-induced limb ische-mia reperfusion injury and the effects of ischemic preconditioning on it.Methods Sixty SD rats ( male and female, 150-200 g) were randomly divided into the control group and the experimental group, each group with 30 rats.Rats in the con-trol group did not receive ischemic preconditioning and just received ischemia for 2, 3 and 4 h, and on day 1, 3, 7 and 14, we took the blood and tested ET level of rats.In the experimental group, after the ischemic preconditioning, on the second day, rats underwent ischemia for 2, 3 and 4 h, and on day 1, 3, 7 and 14, we took the blood and tested ET level of rats. Results Significant difference was found in the plasma ET levels between the two groups on day 1, 3 and 7 at the same processing time, P<0.05 or P<0.01.Significant difference was found in the plasma ET level between the two groups on day 14 at ischemia 3 h, P<0.01.In the experimental group and control group, the plasma ET levels in 1, 3, 7 and 14 days first increased and then decreased, and on day 3 or day 7, it reached the peak (all P<0.05).In the experimental group, significant difference was found in the plasma ET level on day 1, 3, 5 and 7 of different processing time, P<0.05 or P<0.01;in the control group, significant difference was found in the plasma ET level on day 1and 7 of different pro-cessing time, P<0.05 or P<0.01.In the experimental group after ischemia for 2, 3 and 4 h, significant difference was found in the plasma ET level when we compared the different observation time, all P<0.01.In the control group after is-chemia for 2, 3 and 4 h, significant difference was found in the plasma ET level when we compared the different observation time, all P<0.01.Conclusions When the reperfusion injury was induced, the plasma ET of rats reached the peak at the third to seventh day.In the initial stage of limb ischemia reperfusion, the plasma ET level in the blood became higher as the prolonged ischemia time.Ischemic preconditioning could significantly reduce the plasma ET level.
Keywords：ischemia reperfusion injurytourniquetischemic preconditioningendothelin
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 21-23 )