Distribution changes of Vav1 gene E59K site polymorphism in patients with gastric cancer

ZHANG Mingming; TAN Bibo; ZHAO Pei; YU Yueqing; ZHANG Cuigai; LIU Xin

doi:10.3969/j.issn.1002-266X.2016.29.004

Abstract：Objective To investigate the distribution changes of Vav1 gene E59K site polymorphism in the periphreal blood of patients with gastric cancer and its significance.Methods Totally 350 cases of patients with gastric cancer were selected as the gastric cancer group, and 200 cases of healthy people were recruited as the control group.The levels of CEA, FER and CA72-4 were detected in peripheral blood by chemiluminescence.The levels of Vav1 and IL-2 were tested with ELISA.PCR was applied to test E59K site polymorphism of Vav1 gene in the peripheral blood.The various indexes were compared between the two groups, and the relationships of each index with gastric cancer were analyzed.Results Compared with the control group, the levels of Vav1, CEA, FER and CA72-4 in the peripheral blood were higher, whereas the level of IL-2 was lower in the gastric cancer group (all P<0.05).Three kinds of genotypes AA, GA and GG were found for E59K site polymorphism of Vav1 gene.The frequencies of genotypes AA and GA and allelic gene A in the gastric cancer group were higher than those of the control group (all P<0.05).The level of IL-2 gradually decreased in genotypes GG, GA and AA, and the level of Vav1 gradually increased in genotypes GG, GA and AA in the gastric cancer group and the control group (all P<0.05).Logistic regression analysis showed that genotype AA was closely related with progress of gastric cancer, which was an independent risk factor for gastric cancer (OR=2.67, 95%CI:1.814-6.474, P=0.003). Conclusion In the Vav1 gene E59K site polymorphism of patients with gastric cancer, genotype AA is mainly found, which might promote the occurrence and development of gastric cancer by increasing Vav1 expression and decreasing IL-2 expression.