Glycosyl-phosphatidylinositol-B7-1 anchoring into renal carcinoma cell membranes enhances CTL's ability of killing carcinoma cells
GE Huixin
ZHANG Yibing
LI Huizhong
CHANG Jiwu
Abstract：Objective To investigate whether GPI-B7-1 anchoring into the renal carcinoma cell membrane can im-prove the cytotoxic T lymphocyte (CTL)'s ability of killing the cancer cells.Methods We selected the CHO/DHFR-cells that were genetically modified to highly express GPI-B7-1 in liquid nitrogen tanks, then, the target protein GPI-B7-1 was e-luted from the CHO/DHFR-cells and purified.The activity was detected by Western blotting.The renal cell carcinoma cell line CG-6327 was established.We cultured the autologous CTL admixed with GPI-B7-1-CG-6327 membrane, CG-6327 membrane and GPI-B7-1-CHO/DHFR-membrane for 24 hours, and up to now, we got three kinds of CTL.Simple CTL cultured in vitro was taken as the control group, then these four kinds of CTL were added into 96-well plates which were in-oculated with CG-6327 cells for the 24-hour killing experiment.The killing activity of CTL was detected by MTT.Results The killing activity ( A value) of GPI-B7-1-CG-6327 cell membrane-activated CTL was statistically different as compared with that of the control group (P<0.05).No significant difference was found in the CTL cell killing activity activated by others as compared with that of the control group, all P>0.05.Conclusion GPI-B7-1 anchored protein can be trans-ferred and anchored in the cell membrane of RCC surface which provides a second stimulus, and RCC cell membrane with double stimulation signal anchored by GPI-B7-1 has a greater effect for the activation of CTL.
Keywords：kidney neoplasmsGPI-B7-1cytotoxic T lymphocytetumor immunity
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 7-10 )