Protective effect of salvianolic acids on acute liver injury caused by concanavalin A in mice
HE Jiali
LIU Yan
WEN Tao
Abstract：Objective To investigate the protective effect of salvianolic acids on acute liver injury caused by con-canavalin A (ConA) in mice and its mechanism.Methods Male C57BL/6 (B6) mice were randomly divided into four groups:the normal control group, which received saline only; salvianolic acids-treated group, which received salvianolic acids only;ConA-treated group, which was administered with ConA (40 mg/kg) via tail vein injection;and the salvianolic acids+ConA-treated group, which received salvianolic acids at a dose of 50 mg/kg 30 min prior to ConA injection.After 16-hour intervention, the liver function was analyzed by fully automatic biochemical analyser, pathologic changes were ob-served by HE staining, and the relevant inflammatory mediators ( extracellular histones, IL-1 , IL-6, IL-18 and TNF-α) were evaluated by ELISA and Luminex.Results The administration of ConA to mice caused severe liver injury, as evi-denced by significantly increased ALT and AST levels, necrosis and apoptosis of liver cells and inflammatory cell infiltra-tion.Compared with the ConA-treated mice, salvianolic acids plus ConA-treated mice showed a significantly decreasing trend of ALT, AST, as well as the reduced liver injury.Besides, the concentrations of extracellular histones, IL-1 , IL-6, IL-18 and TNF-αwere significantly decreased in the ConA-treated group as compared with those of the normal control group and salvianolic acids-treated group (all P<0.05).Compared with the ConA-treated group, all indexes of the salvianolic acids+ConA-treated group were decreased (all P<0.01), but were higher than those of the normal control group and salvianolic acids-treated group (all P<0.01).Conclusion Salvianolic acids have significant protective effect on ConA-in-duced liver injury in mice, likely due to its ability of decreasing the levels of extracellular histones and various cytokines.
Keywords：liver injurysalvianolic acidsconcanavalin Ainflammatory factors
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 4-6 )