Therapeutic effect and mechanism of resveratrol on human T lymphatic leukemia cells
MA Lijun
JIANG Zhongxing
TIAN Wenliang
HE Chen
GUO Rong
SUN Hui
SUN Ling
LI Yingmei
Abstract：Objective To investigate the therapeutic effect and mechanism of resveratrol (Res) on human T lymphatic leukemia cells (Hut78) .Methods Human T lymphocytic leukemia (Hut78) cells were selected as the target cells, which were treated with 6.25, 12.5, 25, 50, 100 and 200μmol/L Res, respectively.CCK-8 assay was used to detect the cell inhibition rate of Hut78 cells which were treated with 100μmol/L Res for 12, 24 and 48 h, respectively.Flow cytometry was used to detect the apoptosis of Hut78 cells which were respectively treated with 100 and 200μmol/L Res for 12 and 24 h.Western blotting was used to detect the expression of C-myc protein in Hut78 cells which were respectively treated with 100 and 200μmol/L Res for 48 h. Results Resveratrol whose dose was greater than 6.25μmol/L significantly inhibited the proliferation of Hut78 cells, and was time-and dose-dependent (all P<0.01).Res induced the apoptosis of Hut 78 cells, which was dose-dependent (all P<0.01). Res own-regulated the expression of C-myc protein which was in a dose-dependent manner (all P<0.01).Conclusion Res-veratrol has significant anti-leukemia effect on human T lymphatic leukemia, and its mechanism may be associated with the down-regulated expression of C-myc protein.
Keywords：T lymphatic leukemiaresveratrolcell proliferationapoptosisC-myc protein
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 1-3 )