Effects of schisandra chinensis polysaccharide on cell activity of human brain glioma U87 cells

WEN Na; JIN Hong; QI Ling; TANG Zebo

doi:10.3969/j.issn.1002-266X.2016.27.002

Abstract：Objective To observe the effects of schisandra chinensis polysaccharides ( CPSC) on the cell activity of human brain glioma U87 cells, and to explore its possible mechanism.Methods The human glioma U87 cells in the loga-rithmic growth phase were divided into groups A, B, C and control group.The cells in the groups A, B and C were added with the final concentrations of 200, 400 and 800μg/mL of CPSP to culture.The control group was treated with the equal volume of cell culture fluid.The cell viability was observed at 24, 48 and 72 h after culture.At 48 h, the cysteine aspartic acid proteinase 3 (Caspase-3) in the nutrient solution supernatant was detected.Results At 24, 48 and 72 h, the cell survival rates of group A were respectively 1.17 ±0.04, 1.09 ±0.05 and 1.39 ±0.05.The cell survival rates of group B were respectively 1.01 ±0.03, 1.01 ±0.03 and 1.21 ±0.02.The cell survival rates of group C were respectively 0.90 ± 0.02, 0.76 ±0.02 and 0.98 ±0.03.The cell survival rates of the control group were respectively 1.28 ±0.03, 1.16 ± 0.03 and 1.45 ±0.01.At 72 h, the cell survival rate of group B was statistically significant as compared with that of the control group (P<0.05).Significant difference was found in the survival rate between group C and control group at 24, 48 and 72 h (P<0.05).The relative expression of Caspase-3 protein in cultural supernatant of groups A, B, C and control group was respectively 0.79 ±0.02, 0.96 ±0.01, 1.13 ±0.01 and 0.78 ±0.01, and significant difference was found be-tween the groups B, C and the control group (all P<0.05).Conclusion High concentration of CPSC can inhibit the vi-ability of U87 cells, and its mechanism may be that cpsc promote the Caspase-3 expression.