Effects of ramipriI on renaI interstitiaI fibrosis,expression of PDGF-B and CTGF in rats with chronic heart faiIure
ZHOU Jing
YANG Xiaoou
ZHANG Mengyun
LI Xiuhua
XU Jing
XIE Yaqin
Abstract：Objective To observe the effects of ramipril on the renal interstitial fibrosis,expression of platelet derived growth factor (PDGF-B)and connective tissue growth factor (CTGF)in the renal tissues of rats with chronic heart failure. Methods The 30 male Wistar rats were randomly divided into the Sham group (group A,10 rats),model group (group B,10 rats)and the ramipril group (group C,10 rats).The chronic heart failure model was established by the renal upper abdominal aorta coarctation.After the model was successfully established,the rats in the group C were administrated with 1 mg/(kg·d)ramipril for 4 weeks,while rats in groups A and B were administrated with normal saline for 4 weeks.The degree of renal fibrosis was detected under light microscope by HE and Masson staining.The protein and mRNA expression levels of PDGF-B and CTGF were respectively detected by immunohistochemistry and RT-PCR.Results Compared with group A,the renal fibrosis was significantly found in the groups B and C,and group B was more severe than the group C. The protein and mRNA expression levels of PDGF-B and CTGF were significantly higher in the groups B and C than that those of group A,and moreover,group B was higher than group C (all P <0.01).Conclusion Ramipril can effectively decrease the renal interstitial fibrosis,expression levels of PDGF-B and CTGF in rats with chronic heart failure.
Keywords：heart failureramiprilrenal fibrosisplatelet-derived growth factorconnective tissue growth factor
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 10-12 )