Effects of tamoxifen on invasion,activity and expression of MMP-9 in breast cancer MCF-7 ceIIs
CHEN Yan
WANG Jing
XU Yini
HONG Duanyang
PAN Di
SHEN Xiangchun
Abstract：Objective To explore the effects of tamoxifen (TAM)on the invasion,activity and expression of matrix met-alloproteinase-9 (MMP-9)in breast cancer MCF-7 cells,meanwhile to discuss the role of inhibiting G-protein-coupled receptor 30 (GPR30)in these effects.Methods MCF-7 cells in the logarithmic phase were pre-cultured for 24 h in phenol red (PR)-free medium without serum to remove endogenous estrogen before the indicated treatments.MCF-7 cells were seeded in the six-well plates and incubated over night to let them adhere on the plate.The control group was continued to cultivate in PR-free me-dium without serum for 24 h.The TAMgroup was treated with 1 μmol/L TAMin PR-free medium without serum for 24 h.And the TAM+G15 group was pretreated with G15 (1 μmol/L)for 30 min before treatment with TAM(1 μmol/L)for 24 h in PR-free medium without serum.After treatment,cell invasion was detected by Transwell assay.The activity of MMP-9 in culture me-dium was tested by Gelatin zymography assay.The MMP-9 protein expression was analyzed by Western blotting.The mRNA ex-pression of MMP-9 was tested by real-time RT-PCR.Results Compared with control group,the cell invasion was increased, the protein and mRNA expression level of MMP-9 was up-regulated,and the activity of MMP-9 in MCF-7 cells was increased in the TAMgroup (all P <0.05).Furthermore,compared with the TAMgroup,the above indexes of the TAM+G15 group were all decreased (all P <0.05).Conclusions TAMpromotes the cell invasion ability and up-regulates the activity and expression of MMP-9.Meanwhile,the effects may be suppressed by G15 pretreatment.
Keywords：tamoxifenbreast carcinomaG-protein-coupled receptor 30matrix metalloproteinase-9
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 6-9 )