Expression of microproteins in nude mice injected with hepatoma ceII Iines of HepG2,BeI-7402 and Li-7
ZENG Xiaojun
TAO Hualin
WANG Biqiong
YU Hui
Abstract：Objective To investigate the serum proteinogram of hepatoma cell lines (HepG2,Bel-7402 and Li-7) which grow in the nude mice in order to find the microprotein markers which are associated with early diagnosis of hepato-ma.Methods We classified 40 BALB/cA-nu nude mice into 4 groups randomly and marked them as HepG2 group,Li-7 group,Bel-7402 group and control group,respectively.Three types of hepatoma cell lines (HepG2,Bel-7402 and Li-7) were cultured and the cells were prepared into cell suspension (1.5 ×107 /ml),respectively.We injected the cell suspen-sion into the relevant groups with 0.2 mL in every mouse and the control group was injected with RPMI1640 medium. These nude mice were observed for 2 months and we got their blood on the 20th,40th,60th day by eyeball extirpating.We detected the proteinogram using surface enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF-MS)and then,we analyzed the data by Ciphergen ProteinChip 3.0 and Ciphergen Biomaker Wizard 3.1 and SPSS 16.0 to screen the differentiated proteins.Results After the mice in HepG2 group were injected with cell line HepG2,there were 29 differentiated proteins.Among them,8 proteins changed significantly and 5 proteins were up-regulated and 3 were down-regulated.After the mice in Li-7 group were injected with cell line Li-7,there were 12 differentiated proteins.Among them,7 proteins enhanced,and 5 proteins weakened.After the mice in Bel-7402 group were injected with cell line Bel-7402,there were 5 differentiated proteins.Among them,4 proteins enhanced and 1 protein weakened.Conclusions Dif-ferent subtypes of hepatoma cell lines injecting into nude mice can express different microproteins in the serum of mice. These proteins could be identified and detected to be promising markers of different subtypes of hepatoma for the early diag- <br> nosis.
Keywords：liver neoplasmssurface enhanced laser desorption/ionization time of flight mass spectrometryproteomicsdifferential proteinmass-to-charge rationnude mice
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 5-7 )