Effects of free anthraquinone of rhubarb on immune function of mesenteric lymph nodes in rats with severe acute pancreatitis
CHEN Li
FAN Ling
TAN Xiaoyong
WANG Lulu
LIU Yuanqi
XIONG Yuxia
Abstract：Objective To observe the effects of free anthraquinones of rhubarb on immune function in mesenteric lymph nodes (MLN) of severe acute pancreatitis (SAP) rats.Methods Twenty-four healthy male SD rats were randomly divided into the sham operation group , model group, free rhubarb anthraquinone group and rhubarb decoction group , 6 rats in each group.SAP model was made by retrograde pancreatic duct injection of sodium taurocholate in the model group , free rhubarb anthraquinone group and rhubarb decoction group .The sham operation group was injected with equal volume of sterilized normal saline by retrograde injection of pancreatic duct .Rhubarb decoction group and free rhubarb anthraquinone group were respectively administrated by intragastric administration with 200 mg/kg free anthraquinone of rhubarb and 6.6 g/kg rhubarb decoction at 12 hours before and 2 hours after the inducement of SAP .Sham operation group and model group were not treated .MLN was collected 24 hours after molding .The histopathological changes of MLN were observed under light microscope after HE staining .The levels of TNF-αand IL-1 in MLN homogenate were detected by ELISA .The ex-pression of NLRP3 and ASC was tested in the MLN by immunoflurescence .The number of CD4 +, CD4 +CD25+Foxp3+, Th1, Th2, Th1/Th2 ratio and Treg percentage were tested by flow cytometry .Results Lymphoid nodules appeared to be enlarged, cortex was thickened , the quantity of lymphoid nodules was increased in the cortex area of the model group as compared with those of the sham operation group .The quantity and size of lymphoid nodules in the cortex area were smaller in free rhubarb anthraquinone group and rhubarb decoction group than that of the model group .No difference was found in the lymph nodule volume between the free rhubarb anthraquinone group and rhubarb decoction group .Expression level of NLRP3, IL-1 and TNF-α:the model group was significantly higher than the sham operation group (P<0.05), the free rhubarb anthraquinone group and rhubarb decoction group was significantly lower than the model group (P<0.05).CD4 +CD25+Foxp3+, CD4 +and Treg ratio:the model group was significantly higher than the sham operation group (P<0.05), except for CD4 +in the free rhubarb anthraquinone group , the free rhubarb anthraquinone group and rhubarb decoction group was significantly lower than the model group (all P<0.05).Th1, Th2 and Th1/Th2:Compared with the sham op-eration group, Th1 and Th1/Th2 was higher, and the Th2 in the model group was significantly lower (all P<0.05).Th1 and Th1/Th2 was significantly lower in the free rhubarb anthraquinone group and rhubarb decation group than in the model group, and the Th2 in free rhubarb anthraquinone group and rhubarb decoction group was significantly higher than that of the model group (P<0.05).No statistically significant difference was found in the above indicators between the free rhu -barb anthraquinone group and rhubarb decoction group (all P>0.05).Conclusions Free anthraquinone of rhubarb can significantly reduce immune inflammatory response in intestine , and its effect is similar to rhubarb decoction .The mecha-nism may be that free anthraquinone of rhubarb may regulate the body ′s immune balance through inhibiting the expression of inflammasome, regulating Th1/Th2 imbalance and intervening the immune response in early stage of acute pancreatitis .
Keywords：severe acute pancreatitisintestinal immunefree anthraquinone of rhubarbrats
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 13-16 )