Effect of miR-206 on high phosphorus-induced calcification in rat vascular smooth muscle cells
SHAO Juan
WU Jiliang
LI Mincai
Abstract：Objective To investigate the effect of microRNA-206 (miR-206)on β-glycerophosphate (β-GP)-in-duced calcification in rat vascular smooth muscle cells (VSMCs).Methods Aortal VSMCs were primarily cultured and i-dentified in vitro.VSMCs were divided into five groups:control group,mimics negative control group,miR-206 mimic group,inhibitor negative control group and miR-206 inhibitor group.Each group was transfected by lipofectamine2000.Af-ter 48-hour transfection,each group was stimulated with 10 mmol/Lβ-GP to induce calcification of VSMCs.Observing cal-cium deposition with Alizarin red staining while detecting the alkaline phosphate (ALP)activity and the expression of con-nexin 43 (Cx-43)respectively by Trace enzyme standard method and immunofluorescence method after induction for 4 days.Results After transfection 48 h,VSMCs were treated with β-GP for 4 days.Compared with the negative control group,the calcium deposition decreased in the miR-206 mimic group and increased in the miR-206 inhibitor group;the ALP activity and the expression of Cx43 decreased in the miR-206 mimic group and increased in the miR-206 inhibitor group (all P <0.05).Conclusions miR-206 may inhibit β-GP-induced calcification of VSMCs by regulating Cx43.
Keywords：microRNA-206β-glycerophosphatevascular smooth muscle cellscalcificationrats
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 10-12 )