Changes in proliferation and invasive abilities of human chorionic epithelioma cells after silencing Notch1 gene

LIU Shaochen; XU Qian; LI Jianhui

doi:10.3969/j.issn.1002-266X.2016.19.004

Abstract：Objective To observe the changes in the proliferation and invasive abilities of human chorionic epithelio -ma cells after silencing Notch1 gene.Methods We looked up Notch1 sequence (NM 001105721.1) design primers pro-vided by Genbank to synthesize Notch 1 siRNA.Human chorionic epithelioma cells JEG-3 were cultivated and divided into groups A, B and C.The group B was transfected by control siRNA , group C was transfected by Notch1 siRNA and group A without any transfection.12, 24, 36,48, 60 hours after transfection, the cell proliferation ability was determined by MTT in each group , and the invasion ability was detected by Transwell invasive experiment .The expression of MMP-2 and MMP-9 protein was detected by Western blotting 72 h after transfection .Results The cell proliferation ability of group C at 36 h, 48 h and 60 h after transfection was lower than those of groups A and B (all P<0.05).The number of cells penetrating the membrane in the groups A , B and C were 109 ±6 , 118 ±7 and 59 ±4;significant difference was found between group C and groups A and B (all P<0.05).The relative expression of MMP-2 of groups A, B and C was 0.91 ±0.08, 0.87 ± 0.01 and 0.29 ±0.05, respectively.The relative expression of MMP-9 of groups A, B and C was 0.85 ±0.04, 0.79 ± 0.03 and 0.35 ±0.05, respectively.The expression of MMP-2 and MMP-9 in the group C was lower than that of groups A and B (all P<0.05).Conclusion The cell proliferation of human chorionic epithelioma is inhibited and the invasive a-bility is decreased after silencing Notch 1 gene.