Effect of Ginkgo biloba extract on OSAS rats with secondary hypertension

LI Yanhong; CHEN Meixi

doi:10.3969/j.issn.1002-266X.2016.17.005

Abstract：Objective To investigate the effect and mechanism of Ginkgo biloba extract ( GBE) on the obstructive sleep apnea hypoxia syndrome ( OSAS) rats with secondary hypertension.Methods The intermittent hypoxia ( IH) rat models were established.Thirty Wistar rats were randomly divided into the normal oxygen group ( NC group) , IH group and GBE treatment group ( IH+GBE group) .We recorded the changes of blood pressure and physiological statement on week 3, 6 and 9.After 9 weeks, the preadipocytes near kidney of each group were extracted and identified, MTT was used to detect the cell viability and proliferation rates of rat preadipocytes which were cultured with 12.5, 25, 50, 100, 200 and 400 mg/L GBE for treatment of 3, 6, 12 and 24 hours.The preadipocytes in the IH groups were extracted and then were divided into the hypoxia control group, LPS-stimulated hypoxia group ( LPS group) and GBE-treated hypoxia group ( GBE group).After 12 hours, the inflammatory cytokines TNF-α, IL-1βand IL-6 contents in the cell supernatant were detected. Results ①ThebloodpressureoftheIHgroupwasgraduallyincreasedwiththetimeofhypoxiaascomparedwiththatof the NC group (P<0.05 or P<0.01), and the blood pressure in the IH+GBE group maintained normal with the pro-longed time of hypoxia.②GBE could promote adipocyte′s growth with a dose-time relationship (all P<0.05).③ The levels of TNF-α, IL-1βand IL-6 in the LSP group were significantly higher, but those were significantly lower in the GBE group (all P<0.01).Conclusion GBE has therapeutical effect on OSAS-induced hypertension, which may be achieved by promoting the proliferation of preadipocytes, inhibiting the levels of adipocytes and improving the degree of OSAS inflam-mation.