Effects of spirulinakinase on permeability and adhesion molecules of vascular endothelial cells
WANG Huijie
PANG Hui
CHEN Meng
WANG Ke
YANG Ying
CHEN Xiangyi
Abstract：Objective To investigate the effects of spirulinakinase ( SPK) on the cell permeability and the adhesion molecules of vascular endothelial cells, and serum adhesion molecules of atherosclerosis ( AS) rats.Methods The human umbilical vein endothelial cells ( HUVECs) were cultured in vitro, and then were induced by hydrogen peroxide ( H2 O2 ) to establish the oxidative damage endothelial cell models.We determined the permeability rate ( T) of monolayer cell and lac-tate dehydrogenase (LDH) seepage and intercellular adhesion molecule-1 (ICAM-1).The rat AS model was established by high-fat diet, and different doses of spirulina kinase ( SPK) were given by gavage.After 12 weeks, rats were killed and the blood serum was collected to measure ICAM-1 and vascular cell adhesion molecule-1 ( VCAM-1) by ELISA.Results SPK significantly reduced the oxidative damage-induced permeability of monolayer cell, and the leakage of LDH and the secreted ICAM-1 were decreased in vitro experiment (all P<0.05);SPK significantly reduced the levels of ICAM-1 and VCAM-1 in the serum of AS model rats in vivo experiment (all P<0.05).Conclusion SPK can decrease the permeability of vas-cular endothelial cells and reduce the release of adhesion molecules;SPK can decrease the adhesion molecules in serum of AS rats to prevent AS.
Keywords：spirulinakinasevascular endothelial cellslactate dehydrogenaseintercellular adhesion molecule-1vascular cell adhesion molecule-1rafs
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 9-12 )