Expression changes of Id-1 and MMP-9 in colorectal carcinoma tissues and its clinical significance

WU Xueliang; WANG Likun; XUE Jun; YANG Dongdong; QU Ming; GUO Fei; YANG Ruimin; MA Pengcheng; LIU Bo

doi:10.3969/j.issn.1002-266X.2016.15.009

Abstract：Objective To observe the expression changes of inhibitor of DNA binding-1 ( Id-1 ) and matrix metallo-proteinase-9 (MMP-9) in the human colorectal carcinoma tissues and to discuss the clinical significance. Methods The expression of Id-1 and MMP-9 in 50 specimens of colorectal carcinoma tissues ( observation group) and 50 specimens of normal colorectal tissues ( control group) was detected by immunohistochemistry, RT-PCR and Western blotting. The cor-relations between their positive expression and the clinicopathological features were analyzed. Results The positive rates of Id-1 in the observation group and the control group were 72% and 24%, the positive rates of MMP-9 were 78% and 22%, respectively, and there were significant differences between these two groups (all P<0. 05). The positive expression of Id-1 and MMP-9 was related with the depth of tumor invasion, TNM stage, lymph node metastasis, vessel invasion and liver metastasis (all P<0. 05). The relative expression of Id-1 mRNA in the observation group and the control group was 0. 96 ± 0. 03 and 0. 20 ± 0. 04, the relative expression of MMP-9 mRNA was 0. 88 ± 0. 04 and 0. 21 ± 0. 03, respectively, and there were significant differences (all P<0. 05). The relative expression of Id-1 protein was 0. 82 ± 0. 04 and 0. 31 ± 0. 02, the relative expression of MMP-9 in the observation group and the control group was 0. 86 ± 0. 05 and 0. 29 ± 0. 03, respectively, and there were significant differences between these two groups (all P<0. 05). Conclusion The expression of Id-1 and MMP-9 was increased in the human colorectal carcinoma tissues, and the expression was related with tumor in-vasion, TNM stage, lymph node metastasis, liver metastasis and vessel invasion.