Effect of 5-aza-deoxycytidine on proliferation of glioblastoma U87 cells and the mechanism
SHEN Jun
SHAO Xuefei
XU Zonghua
JIANG Xiaochun
XU Shanshui
Abstract：Objective To observe the effect of 5-aza-deoxycytidine on the proliferation of glioblastoma U87 cells and to investigate its possible mechanism. Methods Different concentrations of 5-aza-deoxycytidine (5-aza-deo) (0, 0. 1, 0. 5, 1. 0, 5, 10 μmol/L) were used to interfere in the U87 cell line. After 3 days, the inhibition rate of cell proliferation and the BCL2/adenovirus E1B19kDa protein-interacting protein 3 mRNA (BNIP3 mRNA) expression were compared. Af-ter 5 μmol/L 5-aza-deo was used to interfere in U87 cell line at variant time (0, 1, 2, 3, 4, 5 and 6 d), the inhibition rate of cell proliferation and the BNIP3 mRNA expression were examined. The inhibition rate of cell proliferation was exam-ined after 5 μmol/L 5-aza-deo was used to treat U87 cells for 4 d with Boc-D-FMK or without Boc-D-FMK. Results 5-aza-deo inhibited the U87 cell proliferation which showed a time-and dose-dependent manner (all P<0. 05). The concen-tration with the maximum inhibition was 5 μmol/L. The time of maximum inhibition was the 4th day. Boc-D-FMK did not change the apoptosis of U87 cell induced by BNIP3 (P>0. 05). Conclusion 5-aza-deo can inhibit the U87 cell prolifer-ation, and its mechanism may be related with the up-regulation of BNIP3 mRNA expression.
Keywords：5-aza-deoxycytidineglioblastomaBcl-2/adenovirus E1B19kDa protein-interacting protein 3
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 8-11 )