Effect of fucoxanthin on proliferation and autophagy of human glioma U87 cells
LIU Yugang
SUN Yikun
DAI Yiwu
Abstract：Objective To observe the effect of fucoxanthin on the proliferation and autophagy of glioma U87 cells. Methods The untreated U87 cells were taken as the control group, and U87 cells treated with different concentrations of fucoxanthin were taken as the experimental group. The viability, the apoptosis rate, the number and distribution of autopha-gosome and autolysosome were compared between different groups. Meanwhile, the cell ultrastructure, microtubule-associ-ated protein 1 light chain 3Ⅱ/microtubule-associated protein 1 light chain 3Ⅰ( LC3Ⅱ/LC3Ⅰ) , and Beclin-1 were com-pared between the two groups. Results The cell survival rates of the experiment groups treated with 12. 5, 25, 50 and 75μmol/L fucoxanthin were 93. 19% ± 5. 88%, 73. 05% ± 2. 33%, 49. 95% ± 1. 59% and 25. 95% ± 1. 40%, respective-ly. The viability of the control group was 100. 00% ± 1. 35%. Significant difference was found between the experimental groups treated with 25, 50, 75μmol/L fucoxanthin and the control group (all P<0. 05). The apoptosis rates of the exper-imental groups treated with 25 and 50 μmol/L fucoxanthin were respectively 12. 00% ± 0. 56% and 31. 97% ± 1. 83%, and it was 2. 97% ± 0. 21% in the control group (all P<0. 05). Fucoxanthin induced autophagy in dose-dependent man-ner, which was assayed by transient transfection of LC3-GFP. Compared with the control group, there were more lysosome and autophagolysosomes, indicated by LC3-GFP, in U87 cells treated with fucoxanthin (25 and 50μmol/L). Fucoxanthin-treated cells displayed many lysosome and autophagolysosomes which were observed by transmission electron microscopy. In the experiment groups treated with fucoxanthin ( 25 and 50 μmol/L ) , the expression of LC3Ⅱ/LC3Ⅰand Beclin1 was 0. 86 ± 0. 05, 0. 89 ± 0. 04 and 0. 39 ± 0. 02, 0. 59 ± 0. 04. In the control group, the expression of LC3Ⅱ/LC3Ⅰand Bec-lin1 was 0. 65 ± 0. 01 and 0. 23 ± 0. 01. Compared with the control group, autophagic-related protein expression of LC3Ⅱ/LC3Ⅰand Beclin1 was significantly increased in the experimental groups (all P<0. 05). Conclusion Fucoxanthin can effectively inhibit the proliferation of U87 cells and induce autophagy.
Keywords：fucoxanthingliomamicrotubule-associated protein 1 light chain 3 IImicrotubule-associated protein 1 light chain 3 IBeclin1
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 4-7 )