Effects of FOXA1 on proliferation and cell cycle of human papillary thyroid carcinoma cells
Wang Yuee
Wang Chunyu
Abstract：Objective To observe the effect of forkhead box protein A1 (FOXA1 )on the proliferation and cell cycle of human papillary thyroid carcinoma TPC1 cells and to investigate its mechanism.Methods TPC1 cells were divided into two groups which were separately transfected with Flag-vector (empty vector group)and Flag-FOXA1 (FOXA1 group). The proliferation activity of the two groups of TPC1 cells was detected by MTT method after transfection for 12 h,24 h and 36 h,and cell cycle was detected by flow cytometry at 24 h.The mRNA and protein expression of p27 Kip1 ,cyclin-de-pendent kinase (CDK2)and CyclinD1 was detected by real-time PCR and Western blotting at 24 h after transfection of Flag-FOXA1 .Results In the FOXA1 group,the cell proliferation was promoted,cells in the G1 phase decreased,but cells in the S phase increased.Compared with the empty vector group,the expression of p27 Kip1 mRNA and protein was decreased and the expression of CyclinD1 and CDK2 mRNA and protein was increased.Conclusions FOXA1 can pro-mote the cell proliferation and increase the cells in the S period.The mechanism may be related to the down-regulation of p27 Kip1 and up-regulation of CDK2 and CyclinD1 expression.
Keywords：forkhead box protein A1cell proliferationcell cyclethyroid carcinoma
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 11-13 )