Influence of paracrine effects of bone mesenchymal stem cells on proliferation, apoptosis and secretion function of pancreatic βcell line
XU Yuxin
JING Qingping
ZHAO Cuihong
Abstract：Objective To investigate the influence of paracrine effects of bone mesenchymal stem cells (BMSCs)on the proliferation,apoptosis and insulin secretion of pancreaticβcell line INS-1 cells.Methods The BMSCs were cultured and BM-SCs conditioned media (BMSCs-CM)were obtained by culturing BMSCs with rat pancreatic extracts (RPE)which simulated the pancreatic microenvironment.The INS-1 cells were divided into groups A,B and C,which were cultured with BMSCs-CM,con-ditioned media without RPE and RPMI 1640 culture solution,respectively.The influence of BMSCs-CM on INS-1 cell prolifera-tion was analyzed by MTT.The cell proliferation rate was calculated.The effects of BMSCs-CMon the INS-1 cell apoptosis were measured by AnnexinⅤ/PI double staining.The rate of apoptosis was counted.The insulin concentration of INS-1 cells was ana-lyzed by radioimmunoassay (RIA).Results The cell proliferation rate in the group A was higher than that in group B and C (P<0.05).The apoptosis rate in the group A was lower than that in group B and C (P<0.05).The insulin concentration in the group A was higher than that in group B and C at the concentration of 5.6 mmol/L and 20.0 mmol/L (all P<0.05).Conclu-sions The incubation of BMSCs-CMcan stimulate the INS-1 cell proliferation,inhibit the apoptosis,and promote the insulin se-cretion.The above actions of BMSCs may be achieved by paracrine effects.
Keywords：stem cellsmyeloidpancreatic βcellscytokinesapoptosisproliferation
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 1-3 )