Effect of panax notoginseng saponins in treatment of rats with severe acute pancreatitis

SHENG Guotao; LI Shuangxi; MO Xiaohua

doi:10.3969/j.issn.1002-266X.2016.13.006

Abstract：Objective To investigate the effect of panax notoginseng saponins in treatment of rats with severe acute pancreatitis ( SAP) , and to explore the mechanism.Methods Thirty SD rats were randomly divided into three groups:sham operation group ( SO group ) , severe acute pancreatitis group ( SAP group ) , PNS preconditioning group ( PNS group) , and there were 10 rats in each group.Rat SAP models were induced by retrograde injection of 5%taurocholic acid sodium to pancreatic duct.SO group was just treated with pancreas and duodenum turning after abdominal closure without injection of 5%taurocholic acid sodium.The rats in the PNS group were given intraperitoneal injection of 0.1 mL/100 g PNS 50 mg/mL (xuesetong injection 0.1 mL/100 g) after the models were established.And rats in the other two groups were given normal saline (0.1 mL/100 g) .The serum AMS were detected at 4 hours after modeling, we observed the path-ological changes of pancreatic tissues under the light microscopy.The expression of nuclear transcription factor-κB ( NF-κB) and tumor necrosis factor-α( TNF-α) was detected by immunohistochemical SP method.Results Compared with the group SO, serum AMS levels, pathology scores of pancreatic tissues and the expression of NF-κB and TNF-αwere signifi-cantly increased in the groups PNS and SAP ( all P<0.05) , and those in the SAP group rats were significantly higher than those in the PNS group (all P<0.05).The expression of NF-κB was positively correlated with TNF-αexpression in the pancreas acinar cells (r=0.958, 0.992 and 0.989, all P<0.05).Conclusions Panax notoginseng saponins may reduce the pancreatic injuries of SAP rats.Panax notoginseng saponins may have protective effect on SAP rats by inhibiting the ac-tivation of NF-κB and reducing the excessive release of inflammatory factors.