In vitro toxicity of four kinds of polyphyllins on colon cancer cells

PANG Xiaohui; WANG Chaojie; SHI Zuxuan; CUI Yao; CUI Yongxia; GAO Tianhui

doi:10.3969/j.issn.1002-266X.2016.13.005

Abstract：Objective To investigate the in vitro toxicity of polyphyllins I, V, VI and H on colon cancer cells and to provide guidance for seeking effective chemotherapy drugs of colon cancer.Methods The resurgent SW620, SW480, Co-lo320, Lovo colon adenocarcinoma cell lines were co-cultured with polyphyllin I, polyphyllin VI, polyphyllin H, polyphyl-lin V and oxaliplatin for 48 h respectively, which were then added with CCK-8 for two-hour incubation.The optical density at 450 nm was detected by microplate reader.The cytotoxicity of polyphyllin on colon cancer cells was analyzed by SPSS 23.0.Annexin V/PI staining and flow cytometry was used to detect the apoptotic cells.Western blotting was applied to de-tect Bax and Caspase-3 protein expression.Results The polyphyllin I, polyphyllin VI, polyphyllin H and polyphyllin V showed remarkable antineoplastic activity on SW620, SW480, Colo320 and Lovo cell lines with IC50 of 0.4 mg/L to 8 mg/L.The anticancer activity of polyphyllin I and polyphyllin V were stronger than that of polyphyllin VI and polyphyllin H ( P<0.05).The in vitro anti-tumor effect of polyphyllin I with IC50 of 0.4-0.6 mg/L was the same as oxaliplatin with IC50 of 0.1-2.1 mg/L.Flow cytometry demonstrated that the percentage of Annexin V/PI positive cells treated with polyphyllin I increased 52.3%.Western blotting showing that polyphyllin I mediated expression of Bax and Caspase-3 in colorectal cells was 3 and 1.13 times higher than that of the control group.Conclusion Polyphyllin I, polyphyllin VI, polyphyllin H and polyphyllin V show good anticancer activity against colon adenocarcinoma cell lines in vitro, especially polyphyllin I which can mediate the apoptosis of colon cancer cells.