Effects of baicalein on proliferation, apoptosis and invasion of human colorectal cancer cells
WANG Ning
CHENG Qi
ZHANG Yanxin
Abstract：Objective To investigate the effects of baicalein on proliferation, apoptosis and invasion of human color-ectal cancer cells and to explore the mechanism.Methods Human colorectal cancer cells SW480 in logarithmic phase were randomly divided into the control group, 25, 50 and 100 μmol/L baicalein treatment groups which were respectively treated with normal saline, 25, 50 and 100μmol/L baicalein.The effects of baicalein on proliferation, apoptosis and inva-sion of SW480 cells were detected by EdU, MTT, flow cytometry and Transwell test.The protein expression of Bcl-2, Bax and vascular endothelial growth factor ( VEGF) in SW480 cells after the treatment of baicalein was detected by Western blotting.Results In the 25, 50 and 100μmol/L baicalein treatment groups, the proliferation of SW480 cells was inhibited with a dose and time-dependent manner, the apoptosis rates of SW480 cells were gradually increased, and the invasion a-bilities of SW480 cells were gradually decreased.After the treatment of baicalein, the expression of Bcl-2 and VEGF in SW480 cells was down-regulated, and the expression of Bax was up-regulated (P<0.05).Conclusion Baicalein inhibits the proliferation and invasion of SW480 cells and promots the apoptosis, which might be related with the down-regulation of Bcl-2 and VEGF expression and the up-regulation of Bax expression.
Keywords：colorectal neoplasmsbaicaleincell proliferationapoptosisvascular endothelial growth factor
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 10-12 )