Epicardial adipose tissue volume and MMP-2 and PLA2 levels in epicardial adipose tissues of patients with coronary artery disease

DU Wentao; BAI Jing; ZHI Hua

doi:10.3969/j.issn.1002-266X.2016.12.007

Abstract：Objective To detect the content of epicardial adipose tissue volume ( EATV ) and the levels of matrix metalloproteinase 2 (MMP-2) and phospholipase A2(PLA2) in the epicardial adipose tissues of patients with coronary heart disease ( CHD) and to analyze the significance.Methods A total of 113 patients receiving thoracic surgery were collect-ed, including 67 patients with coronary heart disease (CHD group) and 46 cases with heart valve disease (valvular disease group) .EATV and the nature of the plaque were detected by dual-source CT before surgery to determine whether vascular positive remodeling happened.Then, epicardial adipose tissue ( EAT) and intrathoracic adipose tissue ( TAT) were collect-ed.MMP-2 mRNA and protein levels were detected by RT-PCR and Western blotting.The PLA2 level was detected by ELISA.Finally, the factors related to vascular positive remodeling were analyzed by the logistic regression.Results The EATV in the CHD group was (128.08 ±45.34) cm3 , and (84.21 ±25.37) cm3 in the valvular disease group.There were respectively 36 and 10 cases of vascular positive remodeling in the two groups (all P＜0.01).The MMP-2 mRNA and protein expression levels, PLA2 in EATV of the CHD group were significantly higher than those of the valvular disease group (all P＜0.05), but the differences of the above indexes were not statistically significant in the thoracic adipose tis-sues (all P＞0.05).EATV, MMP-2, PLA2 and high blood pressure were the independent risk factors for the development of vascular positive remodeling in CHD patients.Conclusions The levels of EATV, MMP-2 and PLA2 in the epicardial adipose tissues of CHD patients were increased.EATV, MMP-2 and PLA2 were the independent risk factors for the devel-opment of vascular positive remodeling in CHD patients.