Effect of insulin-like growth factor 1 receptor on cell proliferation and chemotherapy sensitivity of ovarian cancer
LIU Enling
LI Jun
ZHOU Yuxiu
WANG Dehua
MI Ruoran
Abstract：Objective To observe the insulin-like growth factor 1 receptor ( IGF-1R) on cell proliferation and chemo-therapy sensitivity of ovarian cancer.Methods Human ovarian cancer HO8910PM cells were randomly divided into 3 groups.Group A and B were respectively transfected by IGF-1R siRNA and nonsense siRNA, but group C without transfec-tion.The real-time PCR was used to detect IGF-1R mRNA, CCK 8 was employed to detect OD value of cell proliferation, and CCK 8 was used to detect the effective inhibitory concentration ( IC50 ) on cisplatin and carboplatin.Results After transfection for 48, 72 and 96 h, the expression of IGF-1R mRNA in the group A was lower than that of groups B and C (all P<0.05);at 72 h and 96 h, the OD value of group A was lower than those of groups B and C (all P<0.05);at 48 h, IC50 value on cisplatin and carboplatin of group A was lower than those of groups B and C ( all P<0.05) .No significant difference was found between groups B and C ( all P>0.05 ) .Conclusion IGF-1R can promote the cell proliferation, and reduce the chemotherapy sensitivity of ovarian cancer cells to cisplatin and carboplatin.
Keywords：ovarian carcinomainsulin-like growth factor 1 receptorcell proliferationcisplatincarboplatinchem-otherapy sensitivity
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 17-19 )