Influence of sodium ferulate on survival rate of adipose-derived stem cell transplantation and therapeutic effect in mice with hepatic fibrosis
YUE Maoyi
LIU Guangyi
ZOU liyue
HAN Yi
WANG Qiaozhi
Abstract：Objective To observe the influence of sodium ferulate on the survival rate of the transplanted adipose tis-sue-derived stem cells ( ASCs) and the therapeutic effect in mice with hepatic fibrosis.Methods Fifty mice were selected to make the liver fibrosis models through CCl4 injection intraperitoneally for 8 weeks, and then 40 mice with hepatic fibrosis were randomly divided into groups A, B, C and D.Mice in the group A were administered with saline daily by gavage and then injected with cell culture medium by tail vein.Mice in the groups B and D were given 100 mg/kg sodium ferulate by lavage.Mice in the groups C and D received the GFP labeled ASCs (5 ×105 ) injection.The groups B and D were injected to the caudal veins of mice with cell culture medium and were also gavaged with normal saline.Ten mice ( group E) were selected as the control group without any treatment.After 4 weeks, the liver function index was detected, the pathology of liver tissues was observed by Masson staining, the expression of transforming growth factor-β1 ( TGF-β1 ) ,α-smooth muscle actin (α-SMA) in the liver tissues was determined by immunohistochemistry, and the number of ASCs in liver were detec-ted by fluorescence microscope.Results ALT and AST serum levels: group A >groups B, C and D >group E ( P<0.05).ALB serum levels: group E >groups A, B, C and D, group B>group A (P<0.05).Collagen deposition:group A >groups B and C >group D >group E.Expression of TGF-β1 andα-SMA:group A >group B >group C>group D >group E (P<0.05).There was no significant difference in survival rate between group C and group D (P>0.05).Conclusion The combined application of sodium ferulate and ASCs can not significantly increase the survival rate of transplanted ASCs in liver, but has a better therapeutic effect as compared with the separated application.
Keywords：liver fibrosisadipose-derived stem cellsodium ferulatetransforming growth factor-β1
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 10-12 )