Anti-inflammatory effect and mechanism of Kangfuxin in inflammatory response of macrophages
TANG Yanli
ZHANG Yuhao
LI Gang
LI Qiyan
Abstract：Objective To observe the anti-inflammatory effects of Kangfuxin in cell inflammation of macrophages and to discuss the possible mechanism .Methods The cultivated mouse macrophages RAW 264 .7 were randomly divided into 6 groups:group A ( added with DMSO ) , group B ( added with DMSO ) , group C ( added with anti-inflammation drug BAY11-7082), group D (added with 1%Kangfuxin), group E (added with 0.1%Kangfuxin), and group F (added with 0.01%Kangfuxin).We continued to cultivate for 1 h after drug intervention, group A was treaded with DMEM, the other groups were treated with LPS (100 μg/L) to induce cell inflammation.The cells were collected after treatment of 4 h and 8 h, the mRNA expression levels of IL-6, TNF-αand prostaglandin-endoperoxide synthase ( PTGS2) were detected by qRT-PCR.Results Four hours and eight hours after modeling , compared with group A , the mRNA expression levels of IL-6, TNF-αand PTGS2 were increased in the group B (all P<0.05); compared with group B, the mRNA expression levels of IL-6, TNF-αand PTGS2 were decreased in the group C (all P<0.05).All cells were dead in the group D.Com-pared with group B , the mRNA expression of IL-6 and TNF-αwas increased and the PTGS 2 mRNA expression was de-creased in the groups E and F (all P<0.05).Compared with group C, significant difference was found in the mRNA ex-pression of IL-6 and TNF-αbetween groups E and F (all P<0.05), and no significant difference was found in the mRNA expression of PTGS2 between groups E and F (all P>0.05).The concentration and duration of Kangfuxin had no effect on the mRNA expression of IL-6, TNF-αand PTGS2.Conclusion Kangfuxin has inhibitory effect on the cell inflammatory reaction, and its mechanism may be not related with the inhibition of expression of inflammatory factors , such as IL-6 and TNF-α, but be related with the down-regulated PTGS2 expression.
Keywords：inflammationKangfuxinanti-inflammationmacrophagesinflammatory response model
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 13-15 )