Inhibitory effect of phenylacetylglutamine on metastatic lung caner of mice

QUAN Wenqiang; WU Junlu; YAO Yiwen; WAN Haiying; LI Dong

doi:10.3969/j.issn.1002-266X.2016.08.007

Abstract：Objective To observe the inhibitory effect of phenylacetylglutamine ( PG) on lung caner and to investigate the mechanism.Methods The lung cancer metastasis models in C57BL/6 mice was generated by intravenous injection of Lewis lung carcinoma ( LLC) cells.Then the model mice were randomly divided into PG group ( n=20) which was injec-ted with 800 mg/kg PG and PBS group (n=20) which was injected with the same amount of PBS , both for 10 days.We randomly took 5 mice in each group to calculate the tumor count and measure the size of tumor .We randomly took 5 mice in each group to detect the expression of Bcl-2, Bcl-XL, cIAP1, Survivin and PCNA proteins by Western blotting .The broncho alveolar lavage fluid ( BALF ) from 5 mice were collected to detect inflammatory cells and inflammatory factors (TNF-α, IL-6, KC).The electrophoretic mobility shift assay (EMSA) was used to detect the NF-κB DNA binding abili-ty.Results Compared with the PBS group , the growth of lung tumors in mice was inhibited , and tumor number and tumor size decreased significantly in the PG group (P<0.05).The expression of Bcl-2, Bcl-XL, cIAP1, Survivin and PCNA was significantly decreased in the PG group .In BALF of the PG group, binding activity between NF-κB and target DNA was strongly suppressed .Compared with the PBS group , the number of BALF of various inflammatory cells and inflammato-ry cytokines were significantly reduced in the PG group (all P<0.05).Conclusion PG can inhibit the development of lung tumor, and the mechanism may be related with inducing lung tumor cell apoptosis , inhibiting the activation of NF-κB, and reducing the inflammatory reaction.