Gene polymorphism of VEGF and its interaction with smoking in patients with non-alcoholic fatty liver disease
WANG Qian
WU Pengbo
LI Ming
LUO Hesheng
YU Yuanjie
TAN Shiyun
SHU Yongxiang
Abstract：Objective To observe the gene polymorphisms of vascular endothelial growth factor ( VEGF ) rs833061 and rs3025039 and to investigate their interaction with smoking in patients with non-alcoholic fatty liver disease ( NAFLD) . Methods Genotypes of VEGF rs833061 and rs3025039 in 341 patients with NAFLD (NAFLD group) and 246 control subjects ( control group ) were examined by polymerase chain reaction-based restriction fragment length polymorphism ( PCR-RFLP) .The unconditional logistic regression ( ULR) was performed to analyze the relationships between the geno-types and susceptibility to NAFLD .The interactions between genotypes of VEGF rs 833061 and rs3025039 and smoking in inducing NAFLD were explored by generalized multifactor dimensionality reduction ( GMDR) and unconditional Logistic re-gression method.Results The risk of NAFLD in VEGF rs833061 with genotype C (CC+CT) was higher than that of car-riers without C (TT) (all P<0.01), and the risk of NAFLD in rs3025039T gene carriers (TT+CT) was higher than that of carriers without genotype T (CC) (all P<0.01).Gene polymorphism of VEGF rs833061 had interactions with smoking in the process of NAFLD (P<0.01).The risk of NAFLD in rs833061 CC/CT genotype of smoking individuals was 4.93 times higher than that of TT genotype of non-smoking individuals .Conclusions VEGF gene polymorphism is associated with the risk of NAFLD.VEGF rs833061 and smoking have synergistic effects in the process of inducing NAFLD.
Keywords：non-alcoholic fatty liver diseasevascular endothelial growth factorgene polymorphismssusceptibilityinteraction
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 7-9 )