Comparison of expression of TGFβ1 ,α-SMA, smad4 , VEGF and comparison of MVD in different esophageal lesions
XU Zhibin
FENG Junbo
ZHENG Xiuli
WANG Shijie
WU Mingli
Abstract：Objective To detect and compare the changes of transform growth factor β1 (TGFβ1),α-SMA, smad4 protein, vascular endothelial growth factor ( VEGF) and microvessel density ( MVD) in the normal esophageal tissues, low-grade intraepithelial neoplasia ( LGIEN) , high-grade intraepithelial neoplasia ( HGIEN) , early esophageal cancer and ad-vanced esophageal cancer tissues.Methods Twenty cases of normal esophageal tissue specimens ( NC group) , 30 cases of LGIEN specimens ( LGIEN group) , 50 cases of HGIEN specimens, 25 cases of early esophageal cancer specimens ( CIS group) and 25 cases of advanced esophageal cancer specimens (SCC group) were selected.The expression of TGFβ1,α-SMA, smad4 and VEGF protein in each group was detected by immunohistochemistry, and the MVD was determined by Antibody CD34.Results From NC group to SCC group, the positive expression rates of α-SMA, TGFβ1 and VEGF and MVD were gradually increased (all P<0.05);the positive expression rate of smad4 protein was gradually decreased (all P<0.05);and the positive expression rate of TGFβ1 was first increased and then decreased, and the CIS group was the highest (all P<0.05).Conclusions In the course of the development of esophageal cancer from normal esophagus to ad-vanced stage, the phenotype and function of esophageal stromal fibroblasts change, and TGF β1/smad4 protein pathway and angiogenesis may play an important role in the protein pathway.
Keywords：esophageal carcinomaintraepithelial neoplasiatransformling growth factorssmad4 proteinvascular endothelial growth factormicrovessel densityangiogenesis
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 10-12 )