Biological characteristics and resistance mechanism of temozolomide resistance cell line SHG-44/TR
DING Fengxia
ZHU Zhongling
YAN Zhao
Abstract：Objective To investigate the biological characteristics and resistance mechanism of human glioma cell line SHG-44/TR which is resistant to temozolomide ( TMZ) .Methods We induced the human glioma SHG-44 to be re-sistant to TMZ by dose escalation in vitro, and then established the drug-resistant cell line SHG-44/TR.The colony forma-tion assay and MTT colorimetric assay was used to measure the proliferation, resistance and resistance index of SHG-44/TR cells;Western blotting was used to detect the expression changes of resistance protein O6-methylguanine-DNA methyl trans-ferase (MGMT) and human mismatch repair protein 2 (hMSH2);and flow cytometry was used to detect the SHG-44/TR cell cycle.Results After eight months, we successfully established TMZ-resistant glioma cells SHG-44/TR.Significant difference was found in the TMZ-resistance between SHG-44 and SHG-44/TR (P<0.05).SHG-44/TR had no cell cycle arrest phenomenon.The expression of MGMT protein did not change in SHG-44/TR cells as compared with that of parent cells, but the expression of hMSH2 was significantly decreased (P<0.05).Conclusions The hMSH2 expression of TMZ-resistant glioma cells SHG-44/TR is decreased, and the mechanism may be DNA mismatch repair missing.
Keywords：temozolomidedrug-resistance mechanismgliomamismatch repairO6-methylguanine-DNA methyl-transferase
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 19-22 )