Differences between radioresistant cell subline CNE1/70R of nasopharyngeal carcinoma CNE1 and its parental cell line

ZHU Ling; CHENG Jinjian; XU Lingyu; YANG Hua

doi:10.3969/j.issn.1002-266X.2016.05.006

Abstract：Objective To investigate the differences in the doubling time, radiosensitivity, drug sensitivity, apopto-sis and cell cycle distribution between nasopharyngeal carcinoma CNE1 cells and radioresistant cell subline CNE1/70R. Methods Using a radical cure dose (70 Gy) of clinical routine segmentation in vitro irradiation to establish radioresistant nasopharyngeal carcinoma cell line model CNE1/70R.The doubling time of the two cell lines CNE1 and CNE1/70R was measured by cell growth curve.The radiobiological characteristics of the two cell lines were analyzed by the colony forming assay.MTT was used to test the drug sensitivity of the two cell lines to docetaxel and cisplatin.The apoptosis and cell cycle was analyzed by flow cytometry.Results The doubling time of CNE1/70R and CNE1R cells was respectively (1.612 ± 0.069) d and (1.481 ±0.046) d.The result of colony formation assay showed that the radioresistance of CNE1/70R cells was stronger than that of CNE1.Compared with CNE1 cells, CNE1/70R cells were more sensitive to the drugs.After irra-diation, the CNE1 cells blocked in the G2/M phase were more than those of CNE1/70R cells, CNE1 arrested more obvious than CNE1/70R.After the same dose of irradiation, the apoptosis rate of CNE1 cells was higher than that of CNE1/70R cells.Significant differences were found in the above indexes between these two cell lines ( all P<0.05) .Conclusions We successfully establish radiaoresistant nasopharyngeal carcinoma cell subline CNE1/70R, and it has stable differencewith its parental cell line in radiosensitivity and drug sensitivity.