Expression of MMP-9, AECA and ANCA in peripheral blood of patients with Kawasaki disease and its relationship with coronary artery lesions

WANG Xiaohua; WANG Qian; ZHAO Jianmei

doi:10.3969/j.issn.1002-266X.2016.05.004

Abstract：Objective To investigate the potential role and clinical significance of matrix metalloproteinase 9 ( MMP-9), anti-endothelial cell antibodies (AECA) and anti-neutrophil cytoplasmic antibodies (ANCA) in vasculitis and coro-nary artery lesions ( CAL) of patients with Kawasaki disease ( KD) .Methods Forty-two children with KD were divided into two groups:CAL group (n=16) and non-coronary artery lesion (NCAL) group (n=26).Twenty febrile children and 15 children for selective operation were chosen as the control group.Serum levels of MMP-9, AECA and ANCA were meas-ured by enzyme-linked immunosorbent assay ( ELISA) , fluorescence quantitative-PCR and gelatin zymography were respec-tively used to detect MMP-9 mRNA expression and enzyme activity.Results The MMP-9 mRNA expression, protein level and enzymatic activity in the acute phase of KD patients were all higher than those in the two control groups ( all P<0.01), and they were significantly higher in the CAL group than in the NCAL group (all P<0.05).Moreover, MMP-9 mRNA expression, enzymatic activity and protein level were decreased significantly in the remission phase (all P<0.01). Serum AECA and ANCA protein levels of KD patients in the acute phase were significantly higher as compared with those of the two control groups (all P<0.01).The protein level of ANCA in the CAL group was significantly higher than that of the NCAL group (P<0.01).There was a positive correlation between serum MMP-9 and AECA protein in the acute phase of KD (r=0.77, P<0.01).The serum MMP-9 and ANCA protein levels of the CAL group in the acute phase were both positively correlated with LCA/AAO ratio (r=0.57, P<0.05;r=0.88, P<0.01).Conclusions MMP-9, AECA and ANCA are potential pathological factors in the formation of KD vasculitis and CAL.The serum level detection has a certain value for early prediction of CAL.