Effects of baicalein combined with amphotericin B on aspergillus fumigatus biofilm in vitro

LI Bing; CHEN Yiqiang; KONG Jinliang; LUO Jing; DONG Biying

doi:10.3969/j.issn.1002-266X.2016.04.002

Abstract：Objective To investigate the destructive effect and sterilizing effect of baicalein combined with amphoteri-cin B ( AMB) on aspergillus fumigatus biofilm. Methods The broth microdilution method was employed to test the mini-mum inhibitory concentration ( MIC) and minimum fungicidal concentration ( MFC) of baicalein and AMB, and the biofilm models of aspergillus fumigatus were established, and the models were divided into the blank group, baicalein group, AMB group and baicalein+AMB group. After modeling for 24 hours, the corresponding drugs were added to the baicalein group, AMB group and baicalein+AMB group. After 48-hour intervention, crystal violet assay was operated for the semi-quantifi-cation and extracellular matrix was used to observe the biofilm morphology under fluorescence microscopy. Results Crystal violet semi-quantification indicated that there was no significantly difference between AMB group and the control group ( P>0. 05), the biofilm of the baicalein group was significantly lower than that of either AMB group or the control group (P<0. 05), and the baicalein+AMB group was lower than the baicalein group (P<0. 05). Fluorescence microscopy showed that the hypha of control group and AMB group was crossed and encoded with rich extracellular matrix, the hypha of the ba-icalein group was still intensively crossed and became smoother and slender with less extracellular matrix, in the baicalein+AMB group, mycelium was significantly reduced with hardly extracellular matrix. Conclusions Baicalein can destroy the extracellular matrix of aspergillus fumigatus biofilm. Baicalein combined with AMB can increase the penetration of AMB on aspergillus fumigatus and improve its sterilizing effect.