Reversal effect of paeonol on multidrug resistance of liver cancer cell line HepG2/ADM
SUN Aihua
CHEN Jin
GUAN Heng-ming
LI Ping
Abstract：Objective To investigate the reversal activity of paeonol on multidrug resistance of liver cancer cell line HepG2/adriamycin ( ADM) and its potential molecular mechanism.Methods The human HepG2 cell line was used to make the HepG2/ADM model which was induced by the increased concentrations of adriamycin (ADM).MTT assay was performed to detect the proliferation of HepG2/ADM and HepG2.The flow cytometer was used to measure the intracellular accumulation of cytotoxic drugs in HepG2/ADM and HepG2.Western blotting was carried out to determine the levels of P-glycoprotein.Results Paeonol with the concentrations from 0 to 50 μmol/L had no cytotoxicity to the HepG2/ADM and HepG2.The IC50 values for inhibiting proliferation of HepG2/ADM and HepG2 were 1.48 μM and 124.14 μM, and the resistance index was 83.9.Paeonol (25 μmol/L, 50 μmol/L) could significantly enhance the cytotoxic effects of ADM and 5-fluorouracil on HepG2/ADM, and therefore reduced their IC50 values (P<0.01), while It had no effect on those of HepG2.Moreover, Paeonol increased the accumulation of ADM in HepG2/ADM ( all P<0.01) , but not in HepG2.The further study found that Paeonol obviously reduced the expression level of P-glycoprotein in HepG2/ADM (all P<0.01), while had no effect on that of HepG2.Conclusion Paeonol can reverse the multidrug resistance of HepG2/ADM signifi-cantly, which may be related with the inhibition of P-glycoprotein expression and the increased accumulation of cytotoxic drugs.
Keywords：liver neoplasmsHepG2/ADM cell linepaeonolmultidrug resistanceP-glycoprotein
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1-4 )