Effect of γ-ray on wound healing of skin defect in mice and its mechanism
WANG Mu
XU Li
ZHOU Zhi-gang
DONG Zhao-yang
TANG Xue-hong
ZENG Yong
ZHU Li-qian
Abstract：Objective To observe the effect of γ-ray on wound healing of skin defect in mice and to explore its mech-anism.Methods A total of 60 healthy male C57BL/6 mice were randomly divided into the control group (n=30) and observation group (n=30).Those C57BL/6 mice in the observation group were irradiated 5 minutes with a single dose of 60Co-γ-ray (6 Gy).After the mice were anesthetized and sterilized , 1 cm ×1 cm wound to fascia on the back was made , the wound was covered by the TegadermTM paster , and we cleaned the wound every other day .Those mice in the control group were not irradiated , and the other treatments were the same as that in the observation group .The wound residual area was measured using Image analysis system (Image Proplusv 1.5) in the two groups on day 3 (T0), 7 (T1) and 14 (T2) after damage, and the number of new capillaries and fibroblasts in wound were observed at the same time .The mRNA ex-pression levels of vascular endothelial growth factor (VEGF), basic fibroblast growth factor-2 (FGF-2), platelet-derived growth factor ( PDGF) and transforming growth factor-β( TGF-β) in the dermal defects were detected by Real-time PCR. Results With the extension of time , the residual wound areas gradually reduced in the two groups , and the newborn capil-laries and fibroblasts in wound first increased and then decreased .The residual wound areas were larger in the observation group than those of the control group at different time points .The number of new capillaries at T 0 , and the number of fibro-blasts at T0 and T1 in observation group were lower than those of the control group at the same time points (all P<0.05). The mRNA expression levels of VEGF , PDGF and TGF-βat T0 and T1 and the FGF-2 mRNA expression level at each time point in the observation group were lower than those of the control group at the same time points (all P<0.05).Conclu-sion The γ-ray can delay the wound healing of skin defect in mice , and it may be related with the decreased expression of angiogenesis factor and fibroblast factor and inhibition of the angiogenesis and granulation tissue formation .
Keywords：radiation injuryγ-rayskin defectwound healingangiogenic growth factorstransforming growth fac-tor-βmice
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 15-18 )