Bioinformatic analysis of miRNA expression profile in plasma of patients with steroid-induced osteonecrosis of the femoral head
SUN Nan
FAN Yue-guang
LI Peng-fei
ZENG Yi-rong
ZENG Jian-chun
Abstract：Objective To analyze the changes of plasma microRNA expression profile and the content of bioinformat -ics in patients with steroid-induced osteonecrosis of the femoral head ( SONFH ) and to explore its significance . Methods SONFH patients ( observation group ) and healthy volunteers ( control group ) were selected with 6 cases in each group.The real-time PCR was used to detect the relative expression of microRNA in the plasma to screen the differential expression of microRNA .TargetScan , mirBase and miRanda databases were used to predict the target genes which were an -alyzed by gene ontology ( GO) and signal transduction pathway analysis .Results Totally 768 microRNAs were detected by microRNA chip detection .Sixteen microRNAs were different and significant in plasma of SONFH patients .Compared with the control group, 10 were up-regulated (significantly:hsa-miR-1207-5p and hsa-miR-887), and 6 were down-regula-ted (significantly:hsa-miR-96-5p and hsa-miR-576-5p).Totally 887 target genes were screened for the intersection by u-sing TargetScan , mirBase and miRanda three databases .During which , 483 genes were up-regulated , 404 were down-regu-lated.No target genes of hsa-miR-1207-5p were detected, and the target genes of hsa-miR-887 were GSK3A and CASK. Totally 404 microRNAs were down-regulated, 114 Hsa-miR-96-5p-related target genes and 59 hsa-miR-576-5p-related tar-get genes were found .GO and pathway analysis revealed that some differential expression of microRNAs , along with their target genes , were found to be involved in anatomical structure morphogenesis , mesenchymal cell proliferation , positive reg-ulation of metabolic process and significantly enriched in the signal pathway .Conclusions In the plasma of SONFH pa-tients, hsa-miR-1207-5p and hsa-miR-887 were significantly up-regulated, and hsa-miR-96-5p and hsa-miR-576-5p were significantly down-regulated .The abnormal expression of microRNAs in plasma of patients may be involved in the pathogen -esis of SONFH through various signal pathways .
Keywords：osteonecrosis of the femoral headglucocorticoidsmicroRNAgene expression profiling
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1-4 )