Effects of diallyl disulfide on proliferation and invasion of retinoblastoma cells Y79 and the mechanism

LIU Yue-feng; LUO Wei-min; ZHANG Yong; ZHONG Xiao-dong

doi:10.3969/j.issn.1002-266X.2015.47.012

Abstract：Objective To investigate the effects of diallyl disulfide ( DADS) on proliferation and invasion of retino-blastoma cells Y79 and to investigate the mechanism .Methods The retinoblastoma cells Y79 were cultured and divided into the miRNA negative control group ( tansfected by 40 μmol/L scramble ) , miR-222 group ( tansfected by 40 μmol/L miR-222-mimics), DADS negative control group (added with 10 μmol/L DMSO) and DADS group (added with 200μmol/L DADS) and DADS +miR-222 I group ( transfected by 40 μmol/L miR-200 b-inhibitors+200 μmol/L DADS) .The expression of miR-222 treated by different concentrations of DADS in Y 79 cells was detected by qRT-PCR.The prolif-eration and invasion of retinoblastoma cells Y 79 in vitro were determined with MTT and Transwell invasion assay .Results The miR-222 expression of Y79 cells treated with 0, 25, 50, 100, 200 and 400 μmol/L DADS was 2.823 ±0.250, 2.343 ±0.226, 1.955 ±0.267, 1.567 ±0.096, 0.875 ±0.189 and 0.718 ±0.126, respectively.The OD570 of miR-222 was respectively 0.727 ±0.167, 0.949 ±0.070, 0.712 ±0.178, 0.497 ±0.126 and 0.351 ±0.102 in the miRNA nega-tive control group, miR-222M group, DADS negative control group , DADS group and DADS+miR-222I group.Transwell invasion assay results showed that the number of cells permeating the matrigel significantly decreased to 128 ±8 , 179 ±16 , 127 ±12, 76 ±8 and 45 ±14.Significant difference was respectively found between the miR-222M group, DADS group, DADS+miR-222I group and the miRNA negative control group and DADS negative control group (all P<0.05).Conclu-sion DADS can inhibit the proliferation and invasion of retinoblastoma cells Y 79 by down-regulating the expression of miR-222 .