Effects of alcohol extracts from fermented leaves of buckwheat flowers on postprandial blood glucose in rats and its mechanism
ZHANG Ying
ZHANG Sai-hang
CAO Liang-shun
LYU Zhi-wei
HAN Shu-ying
Abstract：Objective To observe the ethanol extracts from fermented leaves of buckwheat flowers ( hereinafter re-ferred to as alcohol extracts ) on postprandial blood glucose in rats and its mechanism .Methods Forty rats were randomly divided into the low-dose, medium-dose and high-dose groups and the control group A (n=10), which were respectively administered by 30, 60 and 120 mg/kg alcohol extracts and purified water .After 20 min, 2.5 g/kg glucose was used for intragastric administration .Meanwhile , the blood glucose was measured at 0 and 60 min.The inhibition rate of blood glu-cose in each group was calculated .We took another 20 rats to kill them to make the everted intestinal sacs .Then we divid-ed the 18 everted intestinal sacs into groups A , B, C, D, E and the control group B , which were respectively placed to 0.5, 1.0, 2.5, 12.5, 25 mg/mL ethanol extracts and purified water .After training for 2 h, we took the liquid within in-testinal sacs to measure the OD value with glucose reagents box and calculated the glucose inhibition rate of each group . Results The blood glucose level at 60 min: the high-dose group <medium-dose group <low-dose group <control group A, and the glucose inhibition rates of the high-dose group, medium-dose group and low-dose group were respectively 17%, 36%and 76%(all P<0.05).The OD values of groups A, B, C, D, E and the control group B were 0.206 ± 0.005, 0.173 ±0.010, 0.159 ±0.013, 0.114 ±0.011 and 0.231 ±0.008 (all P<0.05), and the glucose inhibition rates were 10.8%, 25.11%, 31.17%, 50.65%and 57.14%, respectively (all P<0.05).Conclusions The ethanol extracts may reduce the postprandial blood glucose in GK rats , and the higher the concentration is , the stronger the effect will be, which may be achieved by inhibiting the intestinal absorption of glucose .
Keywords：leaves of buckwheat floweralcohol extractsglucose absorptionpostprandial blood glucose
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 23-25 )