Effect of intracerebroventricular injection of M35 on GLUT4 expression in myocardial tissues of type 2 diabetic rats
ZHANG Zhen-wen
FANG Peng-hua
SHI Min-yi
WANG Yan
CAO Ling
BO Ping
ZHU Yan
Abstract：Objective To observe the influence of intracerebroventricular injection of galanin ( GAL) receptor antago-nist M35 on the expression of glucose transporter type 4 (GLUT4) in myocardial tissues of type 2 diabetic rats.Methods We took 22 healthy male Wistar rats to make the type 2 diabetic models .Sixteen model rats were successfully made and then were divided into the observation group and the model group , 8 rats in each group.Besides, another 8 healthy rats were selected as the control group .The intracerebroventricular injection of 2μL/d M35 was administered in the observation group, and 2 μL/d saline was injected in the model and control groups , once a day and both groups were treated for 21 days.The euglycemic-hyperinsulinemic clamp test was used to detect the glucose infusion rates .GLUT4 mRNA was detec-ted by real-time PCR.Results The glucose infusion rates in the observation group , control group and model group were re-spectively (13.4 ±1.32) mg/min, (22.98 ±2.32) mg/min and (16.91 ±1.56) mg/min, (all P<0.05).GLUT4 mR-NA expression levels in the myocardial tissues of the observation group , control group and model group were 3.72 ±1.79, 5.98 ±0.86 and 11.89 ±2.12, respectively (all P<0.05).Conclusion GAL can enhance the insulin sensitivity of my-ocardial tissues through the increased GLUT 4 expression of type 2 diabetic rats .
Keywords：glucose transporter type 4galanin receptortype 2 diabetes mellitusmyocardial tissueinsulin sensi-tivity
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 8-10 )