Effect of resveratrol on apoptosis of pancreatic βcells and the mechanism
XU Bi-lin
YU Xiu-hua
SHEN Tian
YU Ming
WANG Hong-ping
ZHU Jin-yue
Abstract：Objective To observe the effect of resveratrol on the apoptosis of mice insulinoma cell line β-TC-6 ( hereinafter referred to as βcells) induced by hydrogen peroxide ( H2 O2 ) , and to explore its possible mechanism. Methods The pancreatic βcells were randomly divided into the observation group, control group and blank group, which were respectively cultured in medium with 10 μmol/L resveratrol+200 μmol/L H2 O2 , 200 μmol/L H2 O2 and non-H2 O2 for 48 hours.The apoptosis rate ofβcells and reactive oxygen species ( ROS ) were calculated by flow cy-tometry, superoxide dismutase ( SOD1 ) mRNA was measured by RT-PCR, and the expression of silent information regulator factor 2-related enzyme 1 ( SIRT1 ) and peroxisome proliferator-activated receptor-γcoactivator 1α( PGC-1α) protein was determined by Western blotting.Results Comparison of the apoptosis rate:control group>observa-tion group>blank group ( all P<0.05) , comparison of SOD1 mRNA expression:blank group>observation group>control group (all P<0.05), comparison of ROS levels, control group>observation group>blank group (all P<0.05), and the comparison of SIRT1 protein: blank group>observation group>control group (all P<0.01), the expression of PGC-1αprotein in the observation group and blank group was higher than that of the control group ( all P<0.01).Conclusions Resveratrol significantly reduces the apoptosis of βcells induced by H2O2.The mechanism may be related to the activation of SIRT1/PGC-1αpathways which leads to the increased SOD1 expression and de-creased ROS expression.
Keywords：diabetes mellitusresveratrolreactive oxygen speciessuperoxide dismutasesilent information regula-tor factor 2-related enzyme 1peroxisome proliferator-activated receptor-γcoactivator 1α
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 1-3 )