Changes in concentrations of zinc and PSA EPS of patients withⅢA prostatitis and the clinical significance

CHEN Xi; MO Lin-jian; WANG Xiao-ming; ZHANG Xun; LI Guang-yu; HUANG Shan; JIANG Jie; QIN Chun-ning

doi:10.3969/j.issn.1002-266X.2015.45.007

Abstract：Objective To observe the changes in concentrations of zinc and prostate-specific antigen ( PSA) in the expressed prostatic secretion ( EPS) of patients with ⅢA prostatitis and to investigate the clinical significance .Methods Seventy-one patients with ⅢA prostatitis ( observation group ) and 44 healthy controls ( control group ) were recruited in our study.The concentrations of zinc and PSA in EPS were determined by colorimetric analysis and enzyme -linked immunosor-bent assay (ELISA), respectively.The wet mount was examined promptly with a high-power microscope for WBC in EPS . The pH value of EPS was acquired by pH test strips .Pearson linear correlation analysis was used to determine the correla-tions between zinc concentration , PSA concentration and CPSI scores .The relationships between zinc concentration and pH value, amount of WBC were analyzed by Spearman rank correlation analysis as well as the relationships between PSA con -centration and pH value , amount of WBC .Results The zinc concentrations of the observation group and control group were respectively (155.93 ±68.65) μg/mL and (206.54 ±65.18) μg/mL, and the PSA concentrations were respectively (2.27 ±2.03) ×105 ng/mL and (3.78 ±2.84) ×105ng/mL (all P<0.05).In the observation group, O case of WBC was occasionally found , 32 cases of 1+, 21 cases of 2+, 17 cases of 3+and 1 case of 4+, and WBC of the control Abstract:Objective To observe the changes in concentrations of zinc and prostate-specific antigen ( PSA) in the expressed prostatic secretion ( EPS) of patients with ⅢA prostatitis and to investigate the clinical significance .Methods Seventy-one patients with IIIA prostatitis ( observation group ) and 44 healthy controls ( control group ) were recruited in our study.The concentrations of zinc and PSA in EPS were determined by colorimetric analysis and enzyme -linked immunosor-bent assay (ELISA), respectively.The wet mount was examined promptly with a high-power microscope for WBC in EPS . The pH value of EPS was acquired by pH test strips .Pearson linear correlation analysis was used to determine the correla-tions between zinc concentration , PSA concentration and CPSI scores .The relationships between zinc concentration and pH value, amount of WBC were analyzed by Spearman rank correlation analysis as well as the relationships between PSA con -centration and pH value , amount of WBC .Results The zinc concentrations of the observation group and control group were respectively (155.93 ±68.65) μg/mL and (206.54 ±65.18) μg/mL, and the PSA concentrations were respectively (2.27 ±2.03) ×105 ng/mL and (3.78 ±2.84) ×105ng/mL (all P<0.05).In the observation group, O case of WBC was occasionally found , 32 cases of 1+, 21 cases of 2+, 17 cases of 3+and 1 case of 4+, and WBC of the control group was all occasional .The pH values of the observation group and control group were respectively 6.85 ±0.52 and 6.58 ±0.42 (P<0.05).The concentration of zinc was positively correlated with that of PSA (r=0.330, P<0.001).There were negative correlations between zinc concentration and NIH-CPSI scores including pain scores , quality of life scores and total scores (r=-0.278,-0.224 and -0.248, respectively;all P<0.05).However, zinc concentration was not corre-lated with urinary scores (r=-0.067, P>0.05).Meanwhile, there were negative correlations between PSA concentra-tion and NIH-CPSI scores including pain scores , quality of life scores and total scores ( r =-0.255, -0.217 and-0.250, respectively;all P<0.05);and the PSA concentration was not correlated with urinary scores (r=-0.106, P>0.05).The pH value was negatively correlated with concentrations of zinc and PSA (r=-0.429 and -0.333, respec-tively;all P<0.001).The PSA concentration was negatively correlated with the amount of WBC (r=-0.306, P<0.05).But, there was no correlation between zinc concentration and WBC (r=-0.216,P>0.05).Conclusions The concentrations of zinc and PSA in EPS decreased significantly in patients with ⅢA prostatitis.The detections of concentra-tions of zinc and PSA contributed to the diagnosis and disease evaluation of ⅢA prostatitis.