Association between polymorphisms of F+1 and V4 locus in ADAM33 gene and susceptibility to COPD in Xinjiang Uygur and Han population
WANG Shan
GUAN Jian
AN Rui-fen
WANG Sha-sha
XU Xi-lin
Abstract：Objective To investigate the association between polymorphisms of F +1 and V4 locus in a disintegrin and metalloprotease domain 33 ( ADAM33 ) gene and susceptibility to chronic obstructive pulmonary disease ( COPD ) in Xinjiang Uygur and Han population .Methods The peripheral blood samples from 200 cases of COPD patients and 211 cases of healthy controls in smoking Uygur population and 199 cases of COPD patients and 210 cases of healthy controls in smoking Han population were collected .DNA was extracted .The single nucleotide polymorphism of F +1 and V4 ( ADAM 33 gene) was detected by ABI SNaPshot SNP genotyping .Variance analysis was used to analyze the association between F+1,V4 genotypes in ADAM33 gene and clinical indicators of lung function in patients between Uygur and Han population . Results There were no significant differences in the frequencies of F +1 locus CC, CT, TT genotypes and C, T alleles between the patient group and control group in Xinjiang Uygur and Han population (all P>0.05).There were no signifi-cant differences in the frequencies of V 4 locus GG,CG,CC genotypes and G , C alleles between the patient group and con-trol group in Xinjiang Uygur and Han population (all P>0.05).In the Uygur patient group, there were no significant differences in the percentage of forced expiratory volume in one second (FEV1)/the predicted value (FEV1% pred) and the percentage of forced expiratory volume in one second /forced vital capacity ( FEV1/FVC) between different genotypes of F+1,V4 locus (all P>0.05).In the Han patient group, there were significant differences in the percentage of FEV 1%pred and FEV1/FVC of V4 locus GG genotype (P<0.05).There were no significant differences in three genotypes of F +1 locus and clinical indicators of lung function (all P>0.05).Conclusions There is no significant correlation between the polymorphisms of F+1, V4 locus of ADAM 33 gene and the susceptibility to COPD in Xinjiang Uygur and Han popula-tion.COPD patients with V4 GG genotype have much lower FEV 1%pred and FEV1/FVC than that of the other genotypes in Han population .
Keywords：chronic obstructive pulmonary diseasea disintegrin and metalloprotease domain 33polymorphismsin-gle nucleotideUygur nationalityHan nationalityXinjiang Uygur autonomus region
Publication Date：2015-01-01
Pages：4( 18-21 )