Detection and significance of K-ras and p53 gene mutations in supernatant and sediment of bile in patients with extrahepatic cholangiocarcinoma
ZHANG Shuang-xia
ZHANG Guo-liang
BAO Wen-yi
Abstract：Objective To detect the K-ras and p53 gene mutations both in the supernatant and sediment of bile sam-ples from patients with extrahepatic cholangiocarcinoma ( EHC) and to investigate its significance .Methods Fifty-seven patients with EHC and 42 patients with benign bile duct stricture were surveyed .The supernatant and sediment in their bile was collected by duodenal endoscopy .Polymerase chain reaction-single-strand conformation polymorphism ( PCR-SSCP ) and DNA direct sequencing were performed to detect K-ras mutations and p53 mutations both in the supernatant and sedi-ment of bile samples .Results No gene mutations were detected in 42 patients with benign bile duct stricture in either su-pernatant or sediment of bile .The mutation rate of K-ras gene of patients with EHC was 77.19%(44/57), 66.67%(38/57) in the bile supernatant and 59.65%(34/57) in the corresponding sediment, respectively (P>0.05).The mutation rate of p53 gene of patients with EHC was 70.18% (40/57), 63.16% (36/57) in the bile supernatant and 45.61%(25/57) in the corresponding sediment, respectively (P<0.05).The total mutation rate of combined K-ras and p53 genes in the supernatant and sediment of bile samples from patients with EHC was 85.97%(49/57).Conclusions K-ras and p53 mutations were detected both in the supernatant and sediment of bile from patients with EHC .The combined detec-tion of K-ras and p53 mutations improved the positive rate .The detection of K-ras and p53 mutations may be used in the differential diagnosis of extrahepatic cholangiocarcinoma and benign bile duct stricture .
Keywords：extrahepatic cholangiocarcinomabenign bile duct stricturebilep53 geneK-ras gene
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 4-7 )