Fetus nucleated red blood cell count changes in peripheral blood of pregnant women and its application in prediction of adverse pregnancy outcomes
LIU Li-ping
SHUI Li-jun
GONG Rui-long
CAO Jiang-xia
HUANG Qiu-xiang
ZHANG Yuan-zhen
Abstract：Objective To observe the fetus nucleated red blood cell ( NRBC) count changes in the peripheral blood of pregnant women and to investigate its application in prediction of adverse pregnancy outcomes. Methods Blood samples were obtained from 87 pregnant women with the gestational age from 12 to 18 weeks, peripheral blood mononuclear cells were isolated by density gradient centrifugation and then we analyzed the cells marked with PE-GPA/FITC-CD71 mono-clonal antibodies on the flow cytometer. Tracking the pregnancy outcomes, and their correlation between fetus nucleated red blood cell measurement and adverse pregnancy outcomes was analyzed. Results The percentage of fetus NRBC in women with normal pregnancy outcomes was 0. 89% ± 0. 81%, 3. 25% ± 2. 98% in women with adverse pregnancy outcomes, and there was a significant difference between these two groups(P<0. 05). We took fetus NRBC percentage >3. 79% as the cutoff value to predict adverse outcomes of pregnancy, the sensitivity was 76. 9%, and the specificity was 64. 3%. Conclu-sions Compared with women with normal pregnancy outcomes, women with adverse pregnancy outcomes have a higher a-mount of fetus NRBC in the peripheral blood during the gestational age of 12-18 weeks. Measurement of fetus NRBC in ma-ternal peripheral blood has a predictive role in adverse pregnancy outcomes, with a higher sensitivity and specificity.
Keywords：nucleated red blood cellsfetus nucleated red blood cellspregnancypregnant womenpregnancy outcome
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 34-35,36 )