Anti-tumor effects of tumor cell vaccine by epigenetic modification on pancreatic cancer of hamster
ZHANG Hai-feng
CAO Wei
ZHOU Guo-xiong
CHEN Hai-qin
CHEN Wei-chang
Abstract：Objective To observe the anti-tumor effects of tumor cell vaccine by epigenetic modification on pancreatic cancer of hamster through subcutaneous injection. Methods The hamster pancreatic cancer models were set up by subcu-taneous injection of N-nitrosobis-2-oxopropylamin. We removed the pancreatic tumor, chopped, and took 0. 5 cm × 0. 5 cm × 0. 5 cm tumor tissue to transplant into groin skin of healthy hamsters, and then the hamster subcutaneous transplanted tumor models were set up. In addition, PANC-1 cells were treated by histone deacetylase inhibitors and methyl transferase inhibitor epigenetic modification to make the tumor cell vaccine. Meanwhile, the control cell vaccine was set up. Twelve hamster pancreatic cancer xenografts were divided into two groups, 6 in each group: the vaccinated group and the control group which were respectively treated with tumor cell vaccine and control cell vaccine by subcutaneous injection. The tumor growth and the expression of cyclooxyg enase-2 (COX-2), 5-LOX, GAL-1, GAL-3, MHC Ⅰ and MHC Ⅱ of the two groups were observed. Results In the vaccinated group, the tumor size ( mean size 0. 39 cm) was decreased with the prolonged time, but in the control group, the tumor size ( mean size 0. 82 cm) was increased with the prolonged time. Tumor size was statistical different between the two groups (P<0. 05). The expression of COX-2, 5-LOX, GAL-1 and GAL-3 was lower in the vaccinated group as compared with that of the control group, and MHCⅠ, MHCⅡexpression was higher than that of the control group, the difference was statistically significant (all P<0. 01). Conclusion The epige-netic modification tumor vaccine can effectively delay the growth of transplanted tumor, reduce the expression of COX-2, 5-LOX, GAL-1 and GAL-3, and up-regulate the expression of MHC Ⅰ and MHC Ⅱ.
Keywords：pancreatic neoplasmspancreatic carcinomapancreatic carcinoma cell line PANC-1tumor vaccineepigenetic modificationimmune escapeanimal experiment
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 20-23 )